Recombinant Human Basic Fibroblast Growth Factor
| 【Numbering】BK-NRPA07S | 【CAS】CAS | ||
| 【Item No.】BK-NRPA07S-1 | 【specification】 | ||
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Name: Recombinant human basic fibroblast growth factor
Specification: 10 μg, 100 μg, 1 mg, special order
Product form: freeze-dried powder, the solution before freeze-drying is 5 mM PB (pH 7.4)
Molecular weight: 17.2 kDa
Isoelectric point: 9.58
A280nm (1 mg/mL): 0.945
Source: Escherichia coli
Purity: ≥95% (SDS-PAGE detection)
Endotoxin: <5.0 EU/mg protein
Storage conditions: -20℃ storage
Validity period: 3 years
Activity: EC50=0.25 ~ 1 ng/ml, corresponding activity is 1*106 ~ 4*106 U/mg protein (dose dependent proliferation experiment of mouse BALB/c 3T3 cells)
Related introduction:
Basic fibroblast growth factor (Fibroblast Growth Factor-basic, bFGF), also known as FGF-2, can be combined with heparin. It is a member of the FGF superfamily and is involved in embryo formation, tissue regeneration, wound healing, and central nervous system development. It plays an important role in the process of angiogenesis and tumor development. bFGF is an important mitogenic factor, which is effective for BALB/c 3T3 fibroblasts, capillary and endocardial cells, myoblasts, vascular smooth muscle cells, mesothelial cells, glial and astrocytes, and adrenal cortical cells Mesodermal-derived cells have strong mitogenic and proliferative activity.
Recombinant human bFGF (rh-bFGF) expressed in E. coli is a non-glycosylated single peptide protein containing 155 amino acids and a molecular weight of 17.2 kDa.
Picture 11.jpg
M: protein molecular weight standard
Lane 1: rh-bFGF
Figure 1 SDS-PAGE detection of rh-bFGF
Precautions:
1) After dissolving, place it on an ice bath and try to use it as soon as possible;
2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity
3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;
4) This product is limited to scientific research use.
Figure 2 Detection of proliferation of BALB/c 3T3 cells stimulated by rh-bFGF
Message
Specification: 10 μg, 100 μg, 1 mg, special order
Product form: freeze-dried powder, the solution before freeze-drying is 5 mM PB (pH 7.4)
Molecular weight: 17.2 kDa
Isoelectric point: 9.58
A280nm (1 mg/mL): 0.945
Source: Escherichia coli
Purity: ≥95% (SDS-PAGE detection)
Endotoxin: <5.0 EU/mg protein
Storage conditions: -20℃ storage
Validity period: 3 years
Activity: EC50=0.25 ~ 1 ng/ml, corresponding activity is 1*106 ~ 4*106 U/mg protein (dose dependent proliferation experiment of mouse BALB/c 3T3 cells)
Related introduction:
Basic fibroblast growth factor (Fibroblast Growth Factor-basic, bFGF), also known as FGF-2, can be combined with heparin. It is a member of the FGF superfamily and is involved in embryo formation, tissue regeneration, wound healing, and central nervous system development. It plays an important role in the process of angiogenesis and tumor development. bFGF is an important mitogenic factor, which is effective for BALB/c 3T3 fibroblasts, capillary and endocardial cells, myoblasts, vascular smooth muscle cells, mesothelial cells, glial and astrocytes, and adrenal cortical cells Mesodermal-derived cells have strong mitogenic and proliferative activity.
Recombinant human bFGF (rh-bFGF) expressed in E. coli is a non-glycosylated single peptide protein containing 155 amino acids and a molecular weight of 17.2 kDa.
Picture 11.jpg
M: protein molecular weight standard
Lane 1: rh-bFGF
Figure 1 SDS-PAGE detection of rh-bFGF
Precautions:
1) After dissolving, place it on an ice bath and try to use it as soon as possible;
2) If you cannot use it up at one time, please prepare the mother liquor before use and divide it into small sizes (to ensure the amount of one test) and store it at -65°C to avoid repeated freezing and thawing affecting protein activity
3) Avoid using strong acids, strong bases, strong oxidants, high-concentration organic solvents and other reagents that are likely to cause protein denaturation;
4) This product is limited to scientific research use.
Figure 2 Detection of proliferation of BALB/c 3T3 cells stimulated by rh-bFGF
| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance |
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| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance.The website pictures are from the Internet. The pictures are for reference only. Please take the real object as the standard. In case of infringement, please contact us to delete them immediately. |
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