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Biomedical micro-nanomachines are artificial machines with micrometer or nanoscale features that can perform specific functions in or outside of living organisms, such as detection, imaging, delivery, diagnosis, and treatment, etc. These machines can use a variety of external stimuli, such as magnetic field, light, acoustic waves, temperature, etc., to control their movement direction and speed, and interact with other machines or biomolecules to achieve the identification and intervention of specific goals within the organism. These machines have the advantages of high efficiency, precision, and can be used in a variety of applications in the biomedical field. Case of construction and application exploration of biomedical micro and nano machines: Micro-and nano-robots based on ferrite nanoparticles (FNP) can realize the magnetron movement and communication in the live body. They loaded FNP on different shapes of silicon-based carriers to form different types of micro-and nano-robots, such as disk type, rod type and spiral type. These robots can control their motion direction and speed through an external magnetic field and communicate with other robots or external devices through FNP MR. They have used these robots to perform multiple functions, including drug delivery, imaging, and therapy, in mice. Micro-and nano robots based on DNA origami technology can realize light-controlled motion and intelligent response in the live body. They fold DNA molecules into micro-nano robots with triangular frames, and connect different types of functional molecules at their vertices, such as fluorescent molecules, drug molecules, antibody molecules, etc. These robots can control the direction and speed of their motion through external lasers and achieve intelligent responses with other robots or biological cells through base pairing of DNA molecules. These robots were used to achieve various imaging, diagnostic and therapeutic functions in mice. Micro-nano robots based on metal-organic framework (MOF) can realize sound-controlled motion and intelligent response in living life. They loaded MOF molecules on a silicon-based carrier with hollow structure to form micro-nanorobots with porous properties. These robots can control their motion direction and speed through external ultrasound waves, and achieve an intelligent response to biomolecules through the adsorption properties of MOF molecules. These robots were used to perform drug delivery and treatment in mice.